Counting organisms on the disc using photographs and the Image processing software ImageJ (Fiji)

The following documents are tutorials on how to process photographs of discs using the free software ImageJ / Fiji. These are for more advanced students

Tutorials I to VII each contain a pdf-tutorial and one or more example pictures for practice. (Some images are compressed as zip-files to retain full resolution for analysis.)        

• Introduction to Analysing Fouling on Discs Using ImageJ (110 kB, pdf)
  

 

• Microscopy and ImageJ (73 kB, pdf)
  

 

• I. Manual counting of organisms (13 MB, pdf) + Example Image (11 MB , zip)
  

 

• II. Automated counting of organisms (16 MB, pdf) + Example Image (11 MB , zip)
  

 

• III. Manual measurement of percentage cover (12 MB, pdf) + Example Image (8 MB , zip)
  

 

• IV. Automated measurement of percentage cover (11 MB, pdf) + Example Image (3 MB , tif)
  

 

• V. Simultaneous Analysis of Multiple Discs Using ImageJ (9MB, pdf) + Example Images (480 kB , zip)
  

 

• VI. Counting Organisms Growing on a Disc
  Using ImageJ (Manual): Photomicrographs (10 MB, pdf) + Example Images (290 kB , zip)
  

 

• VII. Determining the sample size (4 MB, pdf) + Example Image (1 MB , tif)